Through the REGIO-MOB project, Italy developed and implemented ECOTRIP -Emission and Consumption Calculation Software based on Trip Data Measured by Vehicle On-Board Unit. ECOTRIP Is a modelling and calculation system to improve the sustainable management and security of the flow of people, vehicles and goods. ECOTRIP calculates the fuel consumption, the emissions of carbon dioxide (CO2) and the main air pollutants produced by combustion of vehicles engines, both during the heating phase of the engine (cold emission) and during the operating temperature (hot emission). This good practice targets the decision-makers involved in policies for mobility and urban traffic. The innovative character and the originality of the software ECOTRIP come from the ability to use data in real time, on the actual paths, on the drive cycle and on the characteristics of the vehicle, as well as operating on different levels of aggregation and detail, according to the journey data made available by the operational centre that controls and manages all on-board units installed in vehicles.